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2.17. Cholyl-lysyl-fluorescein (CLF) transportation
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5 μM CLF (Corning) was incubated with cultures treated with 10 μM Troglitazone (TGZ, Sigma) and 0.02% DMSO vehicle control for 45min. The cultures were then washed five times with PBS and incubated in culture media for 30min before confocal imaging. For CLF quantification, cultures where treated by cell lysis buffer (Sigma) and fluorescence intensity was measured using plate reader.
Copyright and License information: ©2018 The Authors
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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