Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Construction of stable cell lines
Ask a
question
Favorite
To obtain cell lines stably overexpressing UBE2CP3, HepG2 and SMMC7721 cells were infected with Lv-UBE2CP3 and Lv-control viruses (Land, Guangzhou, China). To study the knockdown effects of UBE2CP3 in vitro, HepG2 and SMMC7721 cells were transfected with shRNA-UBE2CP3 (Sh-UBE2CP3) or control (Sh-control) viruses (Obio Technology, Shanghai, China). The infection efficiency was confirmed by qRT-PCR.
Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
