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Population-doubling level (PDL) calculation

SP Stoyan Petkov
TK Tobias Kahland
OS Orr Shomroni
TL Thomas Lingner
GS Gabriela Salinas
SF Sigrid Fuchs
KD Katharina Debowski
RB Rüdiger Behr
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For determining PDL, the fibroblasts were passaged in 10 cm culture dishes (Thermo) with TrypLE at density of 2,5x105 cells /dish. When reaching confluency, cells were harvested and counted before being seeded again at 2,5x105 cells /dish for the next passage. Outgoing from consecutive counts, the PDL was calculated as follows: PDL(n/n-1) = log (Nf/N0)/log 2, where n = passage number, Nf = final number of cells, N0 = number of cells seeded at passage [38].

Copyright and License information:  ©2018 Petkov et al
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