Total NO production in culture medium was determined by measuring the concentration of nitrate and nitrite, a stable metabolite of NO, by modified Griess reaction method. The NO content in each group was detected and calculated according to the instructions of the Total Nitric Oxide Assay Kit.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.