Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Pathway enrichment analysis

AM Agata Michna
US Ulrike Schötz
MS Martin Selmansberger
HZ Horst Zitzelsberger
KL Kirsten Lauber
KU Kristian Unger
JH Julia Hess
request Request a Protocol
ask Ask a question
Favorite

The Reactome pathway database was used to conduct the pathway enrichment analysis in order to further investigate the functions of the selected sets of differentially expressed genes [33]. Only pathways containing not more than 600 genes and not less than 20 genes were considered. Thereby, too general and too specific pathways were excluded from the analysis. Statistical significance of enriched pathways was determined by one-sided Fisher’s exact test. The resulting p-values were adjusted for FDR using the Benjamini-Hochberg method.

Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A