Cell culture

SL Shuangyue LI
HG Huai GUAN
ZQ Zhiqiang QIAN
YS Yijie SUN
CG Chenxue GAO
GL Guixin LI
YY Yi YANG
FP Fengyuan PIAO
SH Shuhai HU
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PC12 cells, a rat pheochromocytoma cell line, possess properties of neurons and exhibit cellular responses to toxicants that are similar to those found in human primary cultures. It has been used extensively as a model for neurotoxicity studies23). In this study, PC12 cells were purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences (Shanghai, China) and cultured in DMEM with 10% fetal bovine serum, 100 U/mlpenicillin and 100 μg/ml streptomycin at 37°C in a humidified atmosphere of 5% CO2.

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