Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
The culture supernatant of the 0.01% HA-treated group (90 μL) was mixed with 10 μL of 100 turbidity reducing units (TRU)/mL hyaluronidase or WFI and incubated overnight at 37 °C, except for the culture supernatant of the 0.01% SI-613-treated group, which was digested by hyaluronidase treatment (30 μL hyaluronidase solution at 60 °C for 3 h). This more aggressive treatment was required because HA in the culture supernatant of the 0.01% SI-613-treated group was more difficult to digest.
Copyright and License information: The Author(s). ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
