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2.8. Colony-Forming Unit (CFU) Assay
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CFU assay was conducted to measure the self-renewal ability of BMSCs with or without DRG cells. Briefly, BMSCs, after coculture with DRG for 8 days, were seeded at the number of 50 cells in a 60 mm plate and cultured for 10 days, then stained with crystal violet staining solution. Colonies containing more than 50 cells were counted under a microscope. Monocultured BMSCs were recognized as the control in all the experiments. All experiments were performed three separate times.
Copyright and License information: ©2018 Shuaishuai Zhang et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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