The viability of AML cells was detected using the Cell Counting Kit-8 (CCK-8, Yiyuan Biotechnology, Guangzhou) according to the manufacturer’s instructions. After indicated treatments, the cells were incubated with CCK-8 reagent for 4 h at 37°C, 5% CO2. The absorbance was measured at a wavelength of 490 nm using a microplate reader (Bio-Tek, VY, USA).
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