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We performed HUVEC migration assay by a transwell chamber with 8 μm pore size (BD Biosciences, USA). The upper chamber was plated with 1 × 105 HUVECs suspended in serum-free DMEM, and the bottom chamber was added with 600 μl hAEC-CM. After 24 hours, we fixed the filters with 10% formalin, stained them with 0.1% crystal violet (Sigma, USA), and then counted the migrated cells by Leica QWin image analysis software. Complete medium was treated as a positive control medium, and serum-free high-glucose DMEM was used as a negative control medium.
Copyright and License information: ©2018 Yongjun Zheng et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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