2.6. Synthesis of the Split-and-Mix One-Bead-One-Compound Peptide Library

The fully randomized peptide library was synthesized on the prepared resin by following ladder synthesis protocol by manually splitting the resin in aliquots. Subsequently, all resin aliquots were mixed for washing, acetylation, and Fmoc deprotection. The cycle of splitting and mixing was repeated until the desired peptide length was accomplished. Finally, the resin was treated with a mixture of 95% trifluoroacetic acid (TFA), 2.5% triisopropylsilane (TIS), and 2.5% deionized water to remove side chain protecting groups, followed by intensive washing with DCM and drying of the resin in vacuo.