Rotenone administration and motor behavior

One week after virus infusion, rats started a 2-week behavioral training period, after which they were given single daily intraperitoneal (IP) injections of 2.8 mg/kg of rotenone suspended in 2% DMSO, 98% Miglyol 812 N as described (Cannon et al., 2009a). Motor deficits were assessed daily using the postural instability test (PIT; Woodlee et al., 2008) until each rat reached its motor behavioral endpoint, defined as the rat’s inability to successfully complete the PIT or loss of 25% body mass (Supplemental Figure 2). Behavioral tests were carried out by investigators blinded to treatment group throughout the study. Animals were euthanized using 0.3 mg/kg pentobarbital, followed by transcardial perfusion and 4% paraformaldehyde perfusion fixation.