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Protein phosphatase 2A methylation level was analyzed indirectly using anti‐demethylated PP2Ac antibody as previously described 13. Briefly, a cell extract sample was divided into several tubes, and one of them was treated with 1 m NaOH to remove methyl groups. Samples were subjected to immunoblotting with anti‐demethylated PP2Ac antibody. Bands of NaOH‐treated samples represent total PP2Ac protein levels, and the level of methylated PP2Ac was calculated as the ratio of band densities normalized with NaOH‐treated samples as 100%.
Copyright and License information: ©2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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