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JEG3 cells were treated as described earlier. Subsequently, cells were washed three times with 500 μl PBS/well. About 800 μl/well MTT solution at 0.5 mg/ml was added to each well, and incubated at 37 °C for 3 h. About 500 μl/well of DMSO was added and plate was wrapped in foil and shaken on an orbital shaker for 15 min. About 200 μl of the resulting solution from each well was transferred from 6-well plates to a well in 96-well plates and the absorbance was measured at 570 nm wavelength.
Copyright and License information: ©2019 The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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