2.4. Trolox Equivalent Antioxidant Capacity (TEAC) Assay

The method was carried out according to the method described by [16] with some modifications to generate the radical cation ABTS · +, 5ml of an aqueous solution of ABTS (7Mm) and 88μl 140mM of potassium persulfate were added, and the solution was incubated at 29°C for 16 hours in the dark. Subsequently, 500 μl of CFH dissolved in phosphate buffer was added to 990 μl of ABTS·+. After 6 minutes the absorbance at 734 nm (6405, JENWAY) was measured. As a positive control, trolox was used, and the TEAC value was calculated from a standard curve using trolox. The values were expressed as mg of trolox per gram of dry extract.