2.10. Flow cytometry analysis of P‐selectin expression

Platelet‐rich plasma was extracted as previously described. 5 μL PRP was added to 45 μL Tyrode's buffer. Platelets were incubated with 5 μL CD41 Allophycocyanin (APC), (Human‐BD, 559777 & Mouse‐ebioscience, 17‐0411‐82) and CD62P Fluorescein (FITC), (Human‐BD, 555523 & Mouse‐Emfret, M130‐1) and incubated at 37°C for 30 minutes ± 30 μmol/L ADP, Dasatinib (LC Laboratories, Woburn, MA, USA) and/or 0.1% DMSO. The reaction was then stopped with the addition of 1% ice cold formalin and analysed on a BD Accuri using C6 software (version 1.0.264.21).