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KS Kaori Sadakane
TI Takamichi Ichinose
MN Masataka Nishikawa
HT Hirohisa Takano
TS Takayuki Shibamoto
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The mice were divided into eight treatment groups (n = 14 per group) as follows: (1) control, (2) ZymA, (3) H-ASD, (4) ZymA + H-ASD, (5) OVA, (6) OVA + ZymA, (7) OVA + H-ASD, (8) OVA + ZymA + H-ASD. The dose of ZymA was 20 ng per mouse, the dose of H-ASD was 0.1 mg per mouse, and the dose of OVA was 4 µg per mouse. ZymA, H-ASD, OVA, and the combinations thereof were dissolved or suspended in 0.1 ml each in sterile Otsuka normal saline (Otsuka Pharmaceutical Co., Ltd., Tokyo, Japan). The mice were intratracheally administrated with the mixed or individual solutions four times at 2-week intervals. Animals in the control group received an intratracheal administration of 0.1 ml of sterile saline.

Copyright and License information: The Author(s) ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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