Human cholangiocarcinoma TFK‐1 and HuCCT‐1 cell lines were maintained in RPMI‐1640 medium containing 10% FBS and 1% penicillin‐streptomycin solution (all from Gibco) in an incubator with 5% carbon dioxide at 37°C.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.