Quantitative RT-PCR assays

JM Jonathan M. Mansbach
PL Pamela N. Luna
CS Chad A. Shaw
KH Kohei Hasegawa
JP Joseph F. Petrosino
PP Pedro A. Piedra
AS Ashley F. Sullivan
JE Janice A. Espinola
CS Christopher J. Stewart
CJ Carlos A. Camargo, Jr.
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By using the NPA samples from hospitalization, quantitative RT-PCR assays were conducted at Baylor College of Medicine, as previously described, for 17 viruses: RSV (types A and B), rhinovirus, influenza (types A and B and 2009 novel H1N1), human metapneumovirus, human bocavirus, parainfluenza viruses (types 1, 2, and 3), adenovirus, enterovirus, and coronaviruses (NL-63, OC-43, HKU1, and 229E).16

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