2.6. Dynamic Light Scattering (DLS) Measurement

Dynamic light scattering (DLS) measurements were performed on a Malvern Nano-ZS ZEN3600 zetasizer (Malvern Panalytical, Malvern, UK). The lyophilized L(D)-NIBC-AuNPs were dissolved in the water with a final concentration of 50 mg·L⁻¹ (volume: 1.5 mL) and then put it into the high-quality quartz cell for testing at 37 °C for 5 times in general mode. To monitor the time-dependent diameters or time-dependent zeta potential of amylin aggregates in presence of L(D)-NIBC-AuNPs, 1.5 mL solution containing 10 μmol·L⁻¹ amylin with the absence or presence of 20 mg·L⁻¹ L(D)-NIBC-AuNPs were added into a high-quality quartz cell (for size) or a DTS1070 folded capillary cell (for Zeta) and then the samples were tested with protein mode continuously for 24 h at 37 °C.