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The level of total glutathione (GSH and GSSG) in the cell was measured using the Glutathione Assay Kit (Sigma-Aldrich, USA) according to the manufacturer’s instructions. Liver samples was firstly deproteinized with 5% 5-sulfosalicylic acid solution and then centrifuged to remove the precipitated protein. Supernatants of the samples were used for the determination of the glutathione according to the manufacturer’s protocol. Samples were read with kinetically at 1 min intervals for 5 min at 412 nm using an ELISA reader (EZ Read 400 Micro-plate Reader, Biochrom).
Copyright and License information: ©2019 Polish Society of Cardiothoracic Surgeons (Polskie Towarzystwo KardioTorakochirurgów) and the editors of the Polish Journal of Cardio-Thoracic Surgery (Kardiochirurgia i Torakochirurgia Polska)
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