The human ovarian cancer cell lines 3AO, HEY, HO8910, SKOV3, and OVCAR3 cells, as well as normal ovarian ISOE80 cells, were purchased from American Type Culture Collection (Manassas, VA, USA). Ovarian cancer lines were maintained in RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS (Thermo Fisher Scientific) and 1% penicillin/streptomycin (Thermo Fisher Scientific). Cells were cultured in a humidified atmosphere containing 5% CO2 at 37°C.
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