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Cell viability measurement by MTT and LDH assay
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For MTT assay, MTT solution was added to the remaining primary microglial and BV-2 microglial cell suspensions and incubated at 37°C for 4 hrs. Absorbance was analyzed at 570 nm using the VICTOR Multilabel Plate Reader. For determining extracellular LDH activity in primary microglial and BV-2 microglial cell culture media, we used a cytotoxicity detection kit according to the manufacturer’s protocol. Absorbance was quantified at 490 nm using the VICTOR Multilabel Plate Reader.
Copyright and License information: ©2019 Park et al.
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