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Apoptosis assay was carried out in both skin sections and H2O2‐treated HUEVC cells using terminal deoxynucleotide transferase dUTP nick end labelling (TUNEL) staining kit (Roche Diagnostics, Indianapolis, IN) according to the manufacturer's instructions. Photographs were taken and visualized with inverted fluorescence microscope (Olympus IX71). The number of apoptotic cells was determined by counting TUNEL and Hoechst double positive nuclei in the field as previously described.23
Copyright and License information: ©2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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