The rat kidneys were removed on day 22 of the treatment. The kidneys were washed with phosphate-buffered saline (PBS), formalin-fixed, embedded in paraffin wax, and sectioned at 2 μm onto glass slides. Sections were de-waxed and stained with hematoxylin and eosin (H&E) for histological examination by light microscopy for the analysis of the renal damage.
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