The DEGs in PDAC samples compared with healthy samples were analyzed with limma. The p-values were calculated by t-test [19] in limma package. BH (Benjamini-Hochberg) [20] was used to adjust p-values into FDR (false discovery rate) values. FDR <0.05 and |log2FC (fold change)| ≥0.58 were set as cutoff criterion for DEGs.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.