Patient specimens were used for EV71 detection and molecular typing. Viral RNA was extracted using magnetic beads (GM-AUTOPREP Kit; Greenmate Co., Seoul, South Korea) according to the manufacturer’s instructions, and the purified viral nucleic acid was processed by using Freedom EVO (Tecan, Männedorf, Switzerland). A highly conserved 5′ noncoding region was the target of a previously described 196-bp region [20]. To determine the EV genotype, conventional reverse transcription polymerase chain reaction (RT-PCR) was carried out for detection of VP1 using primers designed in a previously study [21]. In the ROK, cell culture and RT-PCR were used for identification of EV strains from 1993 to 2004; after 2005, detection of VP1 by RT-PCR was used to genotype EV as a routine detection method, and real-time RT-PCR detection of EV71 was used as the standard detection method since 2008, providing more rapid analysis [22]. The partial VP1 sequences of EV71 have been deposited in GenBank with the accession numbers {"type":"entrez-nucleotide","attrs":{"text":"EU729114","term_id":"190607452","term_text":"EU729114"}}EU729114, {"type":"entrez-nucleotide","attrs":{"text":"EU729123","term_id":"190607470","term_text":"EU729123"}}EU729123, {"type":"entrez-nucleotide","attrs":{"text":"AY125972","term_id":"22656188","term_text":"AY125972"}}AY125972, {"type":"entrez-nucleotide","attrs":{"text":"HM443644","term_id":"309753389","term_text":"HM443644"}}HM443644, HM44366, {"type":"entrez-nucleotide-range","attrs":{"text":"HM443645-HM443662","start_term":"HM443645","end_term":"HM443662","start_term_id":"309753391","end_term_id":"309753425"}}HM443645-HM443662, {"type":"entrez-nucleotide-range","attrs":{"text":"KC162863-KC162883","start_term":"KC162863","end_term":"KC162883","start_term_id":"451788780","end_term_id":"451788817"}}KC162863-KC162883 and {"type":"entrez-nucleotide-range","attrs":{"text":"KT182938-KT182945","start_term":"KT182938","end_term":"KT182945","start_term_id":"953525733","end_term_id":"953525747"}}KT182938-KT182945.
Copyright and License information: The Author(s). ©2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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