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The following chemically synthesized siRNAs were obtained from Dharmacon GE: ON-TARGETplus Non-targeting Control siRNAs #1 (D-001810-01); ON_TARGETplus Human Bmi1 siRNA SMARTpool (L-005230-01). BCBL-1 cells were transfected using the Amaxa Cell Line Nucleofector Kit V (Lonza) according to the manufacturer’s protocol, and cells were lysed at 0, 48, and 96 hours post-transfection in NP-40 lysis buffer for Western blot analysis.
Copyright and License information: ©2018 Hopcraft et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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