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Yarrowia lipolytica strains stored at − 80 °C in 25% glycerol were inoculated into 5 mL YPD medium as the seed culture for 24 h at 30 °C while shaking at 220 rpm, and the culture was transferred to 250-mL shake flasks loaded with 50 mL YPD or YPX medium at an initial optical density at 600 nm (OD600) of 0.5 under the same cultivation conditions for 4 days. All shake flask fermentation experiments were performed in three parallel experimental groups.
Copyright and License information: The Author(s) ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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