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Determination of inflammatory cytokines with ELISA
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The blood of rats was collected and centrifuged at 684 g for 10 min at 4 °C to obtain serum. TNF-α, IL-1β, CRP and IL-6 are bio-markers of lung and systemic inflammation. Levels of TNF-α and IL-1β in BALFs as well as CRP and IL-6 in serum were determined by ELISA according to the instructions supplied by the kit’s manufacturer.
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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