Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Culex Flavivirus (CxFV) strain

CB Cíntia Bittar
DM Daiane Cristina Machado
DV Danila Vedovello
LU Leila Sabrina Ullmann
PR Paula Rahal
JJ João Pessoa Araújo Junior
MN Maurício Lacerda Nogueira
request Request a Protocol
ask Ask a question
Favorite

According to report published previously by this research group [14], The CxFV strain obtained in this study was derived from Culex quinquefasciatus mosquitoes, collected between April 2007 and January 2008, in the city of São José do Rio Preto (SJRP). The mosquitoes were pooled according to genus, gender and geographic location, for RNA extraction using Trizol protocol (Invitrogen).

The presence of Flavivirus was detected for each pool by Multiplex-Nested_RT-PCR [15]. Ten positives pools were inoculated three times passages on C6/36. After the third passage pools were tested with primers specific for Culex Flavivirus (CxFV), Saint Louis Encephalitis Virus (SLEV), and West Nile Virus (WNV) [14]. One CxFV isolated was used for sequence analysis in this manuscript.

Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A