2.5. Catalase Activity Assay:

Thirty minutes after saline vehicle or SA i.p. injection mice were anesthetized with isoflurane, decapitated and hippocampal tissue was isolated and immediately homogenized in 500 ul of buffer containing 50 mmol/L HEPES, 250 mmol/L sucrose, 1 mmol/L EDTA, 0.1% ethanol, and 10% protease inhibitor cocktail (modified from Yakunin et al., 2014) and centrifuged at 18,000 g for one hour at 4°C. Protein was quantified using a Qubit (Thermo Fisher Scientific). Catalase enzymatic activity was measured using an Amplex Red Catalase Assay kit according to the manufacturer’s protocol (Thermo Fisher Scientific).