2.4. α-Glucosidase Inhibition

The in vitroα-glucosidase inhibition assay was performed with an ELISA reader at 405 nm, with acarbose as a specific inhibitor. Briefly, 10 μL of each of MCE, LFE, and MLM at given doses was incubated with a reaction mixture containing 79 μL of sodium phosphate buffer (0.1 M, pH 6.8), 10 μL of para-nitrophenyl glucopyranoside (50 mM), and 1 μL of α-glucosidase (0.5 U/mL) at 37°C for 30 min. After the addition of 200 μL of stop buffer (2 M NaOH), the optical density was measured with an ELISA reader (TECAN, Männedorf, Switzerland) at 405 nm.