To study the expression of TaHsf genes in different organs and response to stress, the wheat expression database (http://wheat.pw.usda.gov/WheatExp/) was used The FPKM (fragments per kilobase of transcript per million fragments mapped) value was calculated for each Hsf gene, the log2 transformed values of the TaHsf genes were used for heat map generation. P-values < 0.05 were taken as statistically significant thresholds [55].
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