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Bacterial strains, plasmids, and growth conditions.

KM Kerrie L. May
KL Kelly M. Lehman
AM Angela M. Mitchell
MG Marcin Grabowicz
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Strains and plasmids used in this study are listed in Tables S1 and S2, respectively, in the supplemental material. Chromosomal mutant alleles were introduced by P1vir transduction. Null alleles were obtained from the Keio collection and their Kanr cassettes were cured using pCP20, as required (71, 72). Spectinomycin-marked ΔcpxR and ΔnlpE alleles have been previously described (44, 45). Strains were grown in Lennox broth (LB) or agar at 37°C. LB was supplemented with ampicillin (Amp; 25 μg/ml), chloramphenicol (Cam; 20 μg/ml), kanamycin (Kan; 25 μg/ml), tetracycline (Tet; 25 μg/ml), spectinomycin (25 μg/ml), and l-arabinose (0.2% [wt/vol]) as required.

Strains used in this study. Download Table S1, DOCX file, 0.1 MB.

Plasmids used in this study. Download Table S2, DOCX file, 0.1 MB.

Copyright and License information:  ©2019 May et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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