Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Fibroblasts were cultured at 2 × 105 cells per well of a six-well plate. The cells were stimulated with 50 ng/ml of recombinant mouse TGF-β1 (R&D Systems), with the control well receiving no stimulant. MSC CM was added to a further well containing TGF-β1 and was cultured for 24 hours. The cells were collected in TRIzol Reagent (Thermo Fisher) and were examined for expression of mouse α smooth muscle actin (α-SMA) by quantitative real-time PCR.
Copyright and License information: ©2016 AlphaMed Press
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
