Mice

Mice (male and female, at least 8 weeks of age) were housed in cages of 5 or 6, maintained on a 12 h light/dark schedule, and allowed ad libitum access to food and water in their home cages. GluA1 S845A knockin mice were obtained from Jackson Laboratory (stock number 012613)^41,42. All of the experiments were conducted according to the Guide for the Care and Use of Laboratory Animals, Japan Neuroscience Society and Tokyo University of Agriculture. All the animal experiments were approved by the Animal Care and Use Committee of Tokyo University of Agriculture (authorization number: 280036). All surgical procedures were performed under Nembutal anesthesia, and every effort was made to minimize suffering. All of the experiments were conducted blind to the treatment condition and/or genotype of the mouse. Animal behavior was recorded using a video camera.