The blood taken from the heart was incubated at 37°C for 15 min and centrifuged at 3000 rpm for 15 min to acquire the serum. The serum samples were kept in a -20°C freezer. Plasma samples were assayed for concentrations of creatinine and BUN using an autoanalyzer (RA 1000; Technicon Instruments, USA).[21]
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