Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Analysis

DF Dorothy Fallows
BP Blas Peixoto
GK Gilla Kaplan
CM Claudia Manca
request Request a Protocol
ask Ask a question
Favorite

Cell supernatants were removed after 48 h (M1) or 24 h (M2) and probed using a multiplex human cytokine/chemokine panel (Bio-Rad, Hercules, CA) according to manufacturer’s instructions. Data/statistical analysis were done as described [16]. Cells were detached with EDTA (20 mM in PBS), washed with PBS, incubated with fluorescently-labeled antibodies against: CCR7, HLA-DR, CD80, CD86, and CD40 (BD Bioscience) (M1) or CD23 (M2), and analyzed on a FACSCalibur (BD Biosciences) using CellQuest (BD Biosciences) and FlowJo (Tree Star) software. Greater than 2,000,000 events per sample were analyzed.

Copyright and License information: Fallows et al. ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A