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1H NMR was used to determine the methacrylation degree of free amine group in GelMA sample [26]. The method was reported as follows: 30 mg GelMA was dissolved in 1 mL D2O to get clear solution. The spectrum was obtained from Advance Bruker 400 M spectrometer. The methacrylation degree of GelMA was calculated as follows:
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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