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Two million cells were suspended in 0.1 ml of saline and injected subcutaneously into 6-week-old female Balb/c nude mice. Four different groups of cells were injected including HCT116, HCT116/LKB1-KO, SW480, and SW480/LKB1-KO, and each group consisted of five animals. Three weeks after tumor cell injection, mice were intraperitoneally injected with Celastrol for 2 weeks (1 mg/kg, three times a week), and sacrificed 1 week after the last Celastrol injection. Tumor xenografts were separated and measured at the end of the experiment.
Copyright and License information: The Author(s), ©2019
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without further permission provided the original work is attributed as
specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
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