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Calcium-independent phospholipase A2 (iPLA2) activity assay
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Control or BME treated Cal27 and JHU022 cell was subjected to iPLA2 activity was measured as described previously [23]. Briefly, cells were washed with ice-cold PBS followed by PLA2 assay buffer, and PLA2 activity in the supernatant was measured at 37 °C for 5 mins, using 100 μM (16:0, [3H] 18:1) plasmenylcholine as substrate.
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