2.2. CAF conditioning medium preparation

Cancer‐associated fibroblasts were grown with complete media in 6‐well dishes for 24 hours to reach 70%‐80% confluency. The 80% confluency cancer cells (MIA PaCa‐2 and Panc‐1) were cultured with 1% FBS DMEM for 24 hours and then changed to complete medium of CAF to cancer cells with 1% FBS culture medium for another 24‐hour culture. After that, the FBS‐free medium culture was followed. After 24‐hour culture, the supernatant was collected and filtered through a 0.2‐μm filter before use. For the CXCL10 or interleukin (IL)‐6 of conditioned medium neutralization, CXCL10 (266‐IP; R&D, Minneapolis, MN, USA) or IL‐6 (7270‐IL; R&D) antibody was added to the conditioned medium with a concentration of 0.2 μg/mL and incubated for 1 hour before use.