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TOP/FOP-Flash luciferase reporter analysis

WH Wenchan Hsu
LL Lifen Liu
XC Xin Chen
YZ Ying Zhang
WZ Weipei Zhu
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TOP/FOP-Flash luciferase reporter assay was used to analyze the activity of Wnt/β-catenin signaling pathway. The vector pRL-SV40 was served as the internal reference. Cells in the control groups were co-transfected with pRL-SV40 and TOP/FOP flash (Promega). Cells in NC groups were co-transfected with pRL-SV40, TOP/FOP flash and si-CASC11 NC/pcDNA 3.1. Cells in si-CASC11 group or pcDNA3.1-CASC11 group were co-transfected with pRL-SV40, TOP/FOP flash and si-CASC11 NC/pcDNA 3.1-CASC11. Dual-Luciferase Reporter Assay System (E1910; Promega) was applied to measure the luciferase activity. The ratio of TOP/FOP indicated the activity of Wnt/β-catenin signaling pathway.

Copyright and License information: The Author(s) ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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