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Haemolytic activity assay

RR Rine Christopher Reuben
PR Pravas Chandra Roy
SS Shovon Lal Sarkar
RA Rubayet-Ul Alam
IJ Iqbal Kabir Jahid
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The method of Maragkoudakis et al. [63] was used to determine the haemolytic activity of LAB isolates. Overnight cultures of LAB isolates grown in MRS broth were streaked onto blood agar base (Diagnostic Pasteur, France) plates containing 5% (v/v) of sheep blood and then incubated at 37 °C overnight. Haemolytic activities of the strains were recorded by the presence of Beta (β) haemolysis (indicated by a clear, colourless/lightened yellow zone surrounding the colonies depicting total lysis of RBC. Alpha (α) haemolysis (indicated by a small zone of greenish to brownish discoloration of the media, depicting reduction of haemoglobin to methemoglobin which diffuses around, and Gamma (ϒ) haemolysis (with no change observed in the media).

Copyright and License information: The Author(s). ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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