AlphaLISA-based demethylase assays

LW Lizhen Wu
JC Jian Cao
WC Wesley L. Cai
SL Sabine M. Lang
JH John R. Horton
DJ Daniel J. Jansen
ZL Zongzhi Z. Liu
JC Jocelyn F. Chen
MZ Meiling Zhang
BM Bryan T. Mott
KP Katherine Pohida
GR Ganesha Rai
SK Stephen C. Kales
MH Mark J. Henderson
XH Xin Hu
AJ Ajit Jadhav
DM David J. Maloney
AS Anton Simeonov
SZ Shu Zhu
AI Akiko Iwasaki
MH Matthew D. Hall
XC Xiaodong Cheng
GS Gerald S. Shadel
QY Qin Yan
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AlphaLISA assays were performed and analyzed as described previously [30] with 25 nM KDM5A (BPS Biosciences, San Diego, CA; 50110), 10 nM KDM5B (1–755) ΔAP [70], 20 nM KDM5B [30, 66], or 25 nM KDM5C (BPS Biosciences, San Diego, CA; 50112).

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