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Cell apoptosis analysis in gefitinib-resistant NSCLC cells was carried out using an Annexin V-FITC apoptosis detection kit (Thermo Fisher Scientific). HCC827/gefitinib cells were washed three times with pre-cold PBS, the cells were then stained with Annexin V-FITC and propidium iodide (PI, Thermo Fisher Scientific) for 20 mins in the dark. The apoptotic cells were counted using a FACS Calibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
Copyright and License information: ©2019 Wang et al.
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