Indomethacin-induced gastric ulcer model

Rats were divided into five groups: control, indomethacin, HemoHIM 250 mg kg⁻¹, HemoHIM 500 mg kg⁻¹, and cimetidine 100 mg kg⁻¹. HemoHIM (250, 500 mg kg⁻¹) dissolved in 0.5% CMC or cimetidine (100 mg kg⁻¹) dissolved in 20% Tween 80, were administered orally after 24 h of fasting. Indomethacin (80 mg kg⁻¹) dissolved in 5% sodium bicarbonate was administered orally 1 h after the administration of HemoHIM or cimetidine. Three hours after administration of indomethacin, rats were anesthetized and euthanized. The inner and outer layers of the gastric wall were fixed with 4% neutral formalin. After 30 min, the stomachs were dissected and washed with PBS.