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Cycloheximide chase experiment

RL Regina Cheuk-Lam Lo
CL Carmen Oi-Ning Leung
KC Kristy Kwan-Shuen Chan
DH Daniel Wai-Hung Ho
CW Chun-Ming Wong
TL Terence Kin-Wah Lee
IN Irene Oi-Lin Ng
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Either Hep3B empty-vector (EV) or Cripto-1 stable-expressing clone was treated with translational inhibitor cycloheximide (CHX, Sigma) at concentration of 50 μg/mL for indicated time. Target protein expression were quantified using ImageJ, which was normalized with respective β-actin levels. Protein level remaining upon CHX treatment was plotted relative to untreated control at 0 h.

Copyright and License information: ADMC Associazione Differenziamento e Morte Cellulare ©2018
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