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Protein-Protein Interaction Assays
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The pull-down assays with GST-Pin1, GST-tagged tau derivatives, His-p53 and His-PARN were performed as described (Cevher et al., 2010; Nazeer et al., 2011; Devany et al., 2013). two-hundred microgram weight of total protein from each NE was pulled-down with 2 μg of the indicated GST- or His-fusion proteins-bound beads, the binding and washing conditions were as described (Cevher et al., 2010; Nazeer et al., 2011; Devany et al., 2013). NEs were treated with 50 mg of RNase A per ml for 10 min at 4°C.
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